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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Ann N Y Acad Sci. 2019 Apr 2;1461(1):53–72. doi: 10.1111/nyas.14037

Figure 3.

Figure 3.

Lipid droplet formation and perilipin expression in beta cell models. (A) Dispersed human islets were stained for insulin (red), neutral lipid (bodipy 493/405, green), and nuclei (DAPI), and the image was captured using a fluorescent microscope using methods published.131 Expression levels of perilipin (PLIN) 1–5 were compared by qPCR using TaqMan primers using methods published131 in human islets from nondiabetic donors (n=7–14) (B), INS1 cells (n=3–4) (C), and islets from a 4-month-old male ad libitum fed C57BL6NJ mouse (n = 6) (D). Data are mean ± SEM and expressed taking the average expression of PLIN2 as 1. N.D., not detected.