Table 2.

Adequacy criteria for rapid-onsite cytologic evaluation

For cytology

>40 lymphocytes/hpf or the presence of germinal center or anthracotic pigment-laden macrophages[83]

>5 fields with at least 100 lymphocytes per low power field (×100) in a smear plus <2 groups of bronchial cells per low-power field (×100) or presence of germinal fragments[85]

Germinal center fragments, >5 fields at×100 magnification with at least 100 lymphocytes per field and <2 groups of contaminating bronchial cells per field[64]

A sequential approach comprising four criteria (core size ≥2 cm, presence of malignant cells, presence of microscopic anthracotic pigments, and mean lymphocyte density ≥40 cells/10 fields (×40)[84]

For molecular testing

A smear with greater than 40% for real-time polymerase chain reaction[54] or 50% tumor cells for NGS[43,62] or ≥ 20% tumor cells[58] for detecting mutation for use of tyrosine kinase inhibitors are required

A minimum of 100 viable tumor cells are required on the stained slide from the cell block. to be tested for PD-L1[44,63]