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. 2020 Jun 18;11:1222. doi: 10.3389/fimmu.2020.01222

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Copyright © 2020 Abbasi Dezfouli, Nikdad, Ghamarnejad, Khajeh, Arefidoust, Mohammadi, Majlesara, Sabagh, Gharabaghi, Kentar, Younsi, Eckert, Poth, Golriz, Mehrabi and Nickkholgh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical scoring after preconditioning with Cyclosporine, Everolimus, and Methylprednisolone 4 h following kidney transplantation. (A1) PS in TNF-α, (A2) IS in TNF-α, (A3) TS in TNF-α, (B1) PS in cytochrome c, (B2) IS in cytochrome c and (B3) TS in cytochrome c. P values were calculated for comparison between intervention groups (Everolimus and Cyclosporine) and Methylprednisolone group. PS and TS were significantly different in TNF-α staining between groups. It shows that apoptosis process was started sooner in Methylprednisolone groups rather than the others.