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. Author manuscript; available in PMC: 2020 Jun 25.
Published in final edited form as: Annu Rev Biochem. 2020 Jun 20;89:45–75. doi: 10.1146/annurev-biochem-013118-111843

Figure 12.

Figure 12.

Products of RNR inhibition experiments depend on whether reductant is used. A F2CDP is a MBI of E. coli and human RNRs in the presence of reductant (protein TR or dithiothreitol) in vitro (70–72), B in cell lines (73), and C in the absence of reductant in vitro. A and C show the products produced during RNR inactivation. B shows SDS-PAGE of RNR from studies in vitro and cell lysate without boiling after incubation with F2CDP (in vitro) or F2C (in cells). Wt-α (80 kDa, C), migrates as 110 kDa.