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. Author manuscript; available in PMC: 2020 Jun 25.
Published in final edited form as: Annu Rev Biochem. 2020 Jun 20;89:45–75. doi: 10.1146/annurev-biochem-013118-111843

Table 1.

Pathway radicals trapped in E. coli class Ia RNR by site-specifically incorporating unnatural amino acids or reaction with N3UDP; PELDOR distances (< ± 1 Å) are given in last column. corresponds to forward RT and to reverse RT, as indicated by the direction of the arrows on the pathway shown below the table.

α β Y122 Y356 Y731 Y730 C439 NDP Y122•-X• distance (Å)
WT WT / - - - - - 33
WT DOPA356 - - - - - 30
WT NH2Y356 - - - - - 30
Y731F F3Y122 - - - - - 30
NH2Y731 WT - - - - - 38
NH2Y731/R411A WT - - - - - 35
NH2Y730 WT - - - - - 39
WT WT - - - - - N3UDP● 48
WT F3Y122 - - - - - 30
WT NO2Y122 - - - - - 30
graphic file with name nihms-1063546-t0002.jpg