Fig 7. Effect of (CA+24-MCA) isolated from hexane extract of F. krishnae on RIN-5F cells by MTT assay.
(CA+24-MCA), cycloartenol (CA) + 24-methylenecycloartanol (24-MCA); values are mean ± S.D.; n = 3; ** P < 0.001 (compared to the same concentration in 5.5 mM glucose); Δ P < 0.05 (compared to control cells cultured in 5.5 mM glucose only); ▪▪ P < 0.001 (compared to control cells cultured in 25 mM glucose only); cell proliferation in normal glucose (5.5 mM) medium treated with 0.1% DMSO was considered as 100%. Percentage cell proliferation in all other doses, viz., (CA+24-MCA) in normal (5.5 mM) and high glucose (25 mM) medium, and control cells (0.1% DMSO) in high glucose (25 mM) medium, were calculated based on their optical densities against those of control cells in normal glucose (5.5 mM) medium.