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. 2020 Jun 25;15(6):e0235220. doi: 10.1371/journal.pone.0235220

Fig 5. Calcium balance in vivo and in vitro.

Fig 5

A) mRNA expression of Ryanodine receptor (RyR1) in tissue homogenates of the superficial and luminal layer of the left ventricle after multiple trauma in pigs. Control/sham animals were presented as black bars, pigs after multiple trauma and nailing (non-reamed), were presented as light grey and pigs after multiple trauma and reaming (reamed) were presented as dark grey bars. B) Sarcoplasmic/ endoplasmic reticulum ATPase (SERCA) mRNA expression after multiple trauma in pigs presented as fold change. C) mRNA expression of natrium calcium exchanger (NCX) presented as fold change. D) RyR1 mRNA expression of HL-1 cells in presence of polytrauma cocktail (PTC). E) SERCA mRNA expression of HL-1 cells incubated with PTC compared to control cells presented as fold change. F) mRNA expression of NCX in HL-1 cells in presence of PTC presented as fold change. Results are significant (*) p<0.05. For statistical analysis one-way ANOVA was used. Graphical presentation as mean ± SEM. For in vitro analysis students t-test was used (in vivo n = 5, in vitro n = 6).