Fig 2. LAMP1⁺-tubule extension from the SCV results from the actions of several effectors.

(A) Comparison of frequency of LAMP1⁺-tubule formation of WT and isogenic multiple-effector deletion mutants in HeLa cells after 8 hours of infection. Cells were fixed at 8 hours post-infection, immunostained, and analyzed as described in the legend of Fig 1. Representative images of select strains are shown. Strain designation (ii, vi, and x) corresponds to strains described in the legend of (B). (B) Quantification of LAMP1⁺-tubule frequency in HeLa cells infected with the multiple-effector deletion mutants for 8 hours. LAMP1⁺-tubule frequency was quantified and analyzed as described in the legend of Fig 1. Strain legend: “+” = gene present, “-” = gene deleted. A “+” for all genes indicates wild type (strain i). Results analyzed by a Kruskal-Wallis test with Dunn’s correction for multiple comparisons. All LAMP1⁺-tubule frequencies in the multiple-deletion mutant strains were significantly different from wild type, however there was no significance between the multiple-deletion mutants themselves.