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. 2020 Jun 25;15(6):e0235020. doi: 10.1371/journal.pone.0235020

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© 2020 Knuff-Janzen et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — HeLa cells were infected with the indicated S. Typhimurium strains for 8 hours, fixed, and immunostained for Salmonella (red) and Golgin-97 (green), and the nucleus was stained with DAPI (blue). (A) Quantification of S. Typhimurium position relative to the Golgi. The distances from the center of individual bacteria to the nearest edge of the Golgi complex was measured in infected cells. Strain legend: “+” = gene present, “-” = gene deleted. All data points are shown to accurately indicate the spread of the data. The averages for three separate experiments are shown (n = 3). An asterisk indicates a significant difference (p < 0.003) between the indicated mutant strain and the corresponding WT strain or other strain if indicated by as determined by a Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison post-test. ns = not significant. (B) Select representative images used to enumerate distances in (A). (C) SL1344 strain ΔsseFGΔsteAΔpipB2 was complemented with a low-copy plasmid expressing a functional copy of PipB2. HeLa cells were infected, fixed, stained, and analyzed as described in (A).

Inline graphic — HeLa cells were infected with the indicated S. Typhimurium strains for 8 hours, fixed, and immunostained for Salmonella (red) and Golgin-97 (green), and the nucleus was stained with DAPI (blue). (A) Quantification of S. Typhimurium position relative to the Golgi. The distances from the center of individual bacteria to the nearest edge of the Golgi complex was measured in infected cells. Strain legend: “+” = gene present, “-” = gene deleted. All data points are shown to accurately indicate the spread of the data. The averages for three separate experiments are shown (n = 3). An asterisk indicates a significant difference (p < 0.003) between the indicated mutant strain and the corresponding WT strain or other strain if indicated by as determined by a Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison post-test. ns = not significant. (B) Select representative images used to enumerate distances in (A). (C) SL1344 strain ΔsseFGΔsteAΔpipB2 was complemented with a low-copy plasmid expressing a functional copy of PipB2. HeLa cells were infected, fixed, stained, and analyzed as described in (A).