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. 2020 Jun 25;15(6):e0235020. doi: 10.1371/journal.pone.0235020

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© 2020 Knuff-Janzen et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Strain legend: “+” = gene present, “-” = gene deleted. A “+” for all genes indicates wild type. (A) Replication of S. Typhimurium single- and sequential- deletion mutants in RAW 264.7 macrophages. RAW 264.7 cells were infected with the indicated strains at a MOI of 10. Fold change was determined by dividing CFU counts at 24 hours post-infection by CFU counts at 2 hours post-infection. The average fold change ± standard deviation for three experiments is shown (n = 3). An asterisk indicates a significant difference (p < 0.02) between the indicated mutant strain and WT or other strain if indicated by as determined by a Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison post-test. ns = not significant. (B) Replication of S. Typhimurium multiple-effector deletion mutants in RAW 264.7 macrophages. Experiment was performed and analyzed as described in (A). (C) Replication of S. Typhimurium strains in THP-1 monocytes. THP-1 monocytes were infected with select S. Typhimurium strains and results analyzed as described in (A). Representative results from one experiment is shown. An asterisk indicates a significant difference (p < 0.03) between the indicated mutant strain and WT or other strain if indicated by as determined by a Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison post-test. ns = not significant.

Inline graphic — Strain legend: “+” = gene present, “-” = gene deleted. A “+” for all genes indicates wild type. (A) Replication of S. Typhimurium single- and sequential- deletion mutants in RAW 264.7 macrophages. RAW 264.7 cells were infected with the indicated strains at a MOI of 10. Fold change was determined by dividing CFU counts at 24 hours post-infection by CFU counts at 2 hours post-infection. The average fold change ± standard deviation for three experiments is shown (n = 3). An asterisk indicates a significant difference (p < 0.02) between the indicated mutant strain and WT or other strain if indicated by as determined by a Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison post-test. ns = not significant. (B) Replication of S. Typhimurium multiple-effector deletion mutants in RAW 264.7 macrophages. Experiment was performed and analyzed as described in (A). (C) Replication of S. Typhimurium strains in THP-1 monocytes. THP-1 monocytes were infected with select S. Typhimurium strains and results analyzed as described in (A). Representative results from one experiment is shown. An asterisk indicates a significant difference (p < 0.03) between the indicated mutant strain and WT or other strain if indicated by as determined by a Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison post-test. ns = not significant.