Table 2. Plasmids used in this study.
Plasmid Designation | Relevant Characteristics/Genotype | Source/Reference |
---|---|---|
pRE112 | cat sacB oriVRGKγoriTRP4 CmR | [33] |
pACYC184 | oriP15A, TetR, CmR | [79] |
pRE112-ΔsseFΔsseG | Upstream region of sseF and downstream region of sseG from S. Typhimurium SL1344 in pRE112 | This study |
pRE112-ΔsteA | Upstream and downstream regions of steA region from S. Typhimurium in pRE112 | This study |
pRE112-ΔpipB2 | Upstream and downstream regions of pipB2 region from S. Typhimurium in pRE112 | This study |
pRE112-ΔsopD2 | Upstream and downstream regions of sopD2 region from S. Typhimurium in pRE112 | This study |
pRE112-ΔsseJ | Upstream and downstream regions of sseJ region from S. Typhimurium in pRE112 | This study |
pPIPB2 | pipB2 under the control of its native promoter in pACYC184 | This study |
CmR = Chloramphenicol resistance, TetR = Tetracycline resistance