. 2020 Jun 25;15(6):e0235020. doi: 10.1371/journal.pone.0235020

Table 3. Primers used in this study.

	Forward oligonucleotide	Reverse Oligonucleotide
sseF 5’ flanking	`GAACTGCATGAATTCCCGGGCTGGACAGTTTTATCCGCCG`	`CGGTATATACCTGAAAACGATTACATATTTCGTTCTGTTATTTAAGCAATAAG`
sseG 3’ flanking	`GAAATATGTAATCGTTTTCAGGTATATACCGG`	`CAAGCTTCTTCTAGAGGTACCGAAATAACAGACGCAGCGCC`
steA 5’ flanking	`GAACTGCATGAATTCCCGGGCCATCGCTTTGTGATACCCC`	`CATATCCTACTCCTTCAAATTTTGCTC`
steA 3’ flanking	`CAAAATTTGAAGGAGTAGGATATGTAAAAAGCGTTTATGTTTAGCC`	`CAAGCTTCTTCTAGAGGTACCCGGGATGAGACAGAATGACC`
pipB2 5’ flanking	`GAACTGCATGAATTCCCGGGGCTGCATCGTCATACTACGG`	`CATATATTTTCTCCCAGAGACAGCAAC`
pipB2 3’ flanking	`GTCTCTGGGAGAAAATATATGTAGCCCTTTTTGACGTAAATCTG`	`CCAAGCTTCTTCTAGAGGTACCCCTGGTAATATTTATCAGGCG`
sopD2 5’ flanking	`GTGAACTGCATGAATTCCCGGGGGGGTTTATGGACACATTCC`	`CTTTTTACATAATAACTCCCTTGATTATTTACCG`
sopD2 3’ flanking	`GTAAATAATCAAGGGAGTTATTATGTAAAAAGTCATTAAAAAGGCC`	`CAAGCTTCTTCTAGAGGTACCGTTCTGACCATTACTTCTAACG`
sseJ 5’ flanking	`CAAGCTTCTTCTAGAGGTACCCCCACTCCCCACGCTATTATG`	`CATAGTGTCCTCCTTACTTTATTAAACACG`
sseJ 3’ flanking	`CGTGTTTAATAAAGTAAGGAGGACACTATGTAAAGTTCCATCGGCTGCGG`	`ATGAATTCCCGGGAGAGCTCCCTGGCAACGGTTAAGGTGG`
sifA 5’ flanking	`CAAGCTTCTTCTAGAGGTACCCACCCCGAGCGCCGTTATTATC`	`CGTCTGATTTTACATATTAATCTCACTTATACTGGAG`
sifA 3’ flanking	`GAGATTAATATGTAAAATCAGACGACGCTTTCTCAGACG`	`ATGAATTCCCGGGAGAGCTCGACCGTGACGACCACAAACG`
pRE112 plasmid backbone	`GGTACCTCTAGAAGAAGCTTGGGA`	`CCCGGGAATTCATGCAGTTCAC`
pACYC184 plasmid backbone	`GCGGCCGCTCGATACCCATACG`	`CCCGAGATGCGCCGCGTGC`
pipB2 complementation	`GCACGCGGCGCATCTCGGGGAGTTGCAGGAAGGCGGCAAGC`	`GTATGGGTATCGAGCGGCCGCAATATTTTCACTATAAAATTCGTTAAAGAGTG`