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. 2020 Jun 15;16(6):e1008554. doi: 10.1371/journal.ppat.1008554

Fig 2. ATGL and G0S2 colocalize at the lipid droplet surface.

Fig 2

ATGL and G0S2 were expressed by lentiviral transduction and detected by indirect immunofluorescence using antibodies against the respective epitope tag. Colocalization between the proteins or with the lipid droplet markers (BODIPY 493/503 or AUTOdot [42]) was tested in Lunet N hCD81 cells cultured with or without oleic acid (OA), as indicated. (a) Subcellular localization of HA-tagged ATGL WT or S47A relative to lipid droplets. (b) Subcellular localization of Flag-tagged G0S2 relative to lipid droplets. Note that we had to co-transduce ATGL (here HA-tagged ATGL, which was left unstained) to achieve a robust and reproducible G0S2 detection. (c) Subcellular localization of Flag-tagged G0S2 relative to HA-tagged ATGL and lipid droplets. The plots on the right side indicate the intensity profiles in the different channels along the red dotted line depicted in the merge picture.