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. 2020 Jun 15;16(6):e1008554. doi: 10.1371/journal.ppat.1008554

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© 2020 Vieyres et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — (a) Immunofluorescence analysis of HA-tagged ATGL localization respectively to the lipid droplets (BODIPY 493/503) and trans-Golgi (p230 marker) in Lunet N hCD81 cells. (b) Intensity profiles of the green and red channels along the dotted line depicted in the merge pictures of panel a. (c) Quantification of ATGL accumulation in the nucleus, the cytoplasm and at the lipid droplet periphery. The heatmap shows the fold enrichment of the protein signal in each compartment as compared to the mean protein signal in the whole cell. Average of 3 experiments with at least 10 pictures per experiment.