Table 1. Oligonucleotide primers and probes for qPCR assays for the detection of A. caninum, A. ceylanicum, A. braziliense, and U. stenocephala.
| Target | Primers and probes | Sequence (5’–3’) | Target | Size (bp) |
Conc. nm | Source |
|---|---|---|---|---|---|---|
| Hookworms | ACancey F | GGG AAG GTT GGG AGT ATC G | ITS-1 | 103 | 300 | This study |
| AcanceyR | CGA ACT TCG CAC AGC AAT C | 300 | This study | |||
| A. caninum | Acan probe | 5HEX/AG+T+CGT+T+A+C+TGG/3IABkFQ | 100 | This study | ||
| A. ceylanicum | AceyDOGprobe | 5Cy5/CCGTTC+CTGGGTGGC /3lAbRQSp | 100 | (Hii et al., 2018) | ||
| Hookworms | UncbrazF | GAG CTT TAG ACT TGA TGA GCA TTG | ITS-1 | 700 | This study | |
| UncbrazR | GCA GAT CAT TAA GGT TTC CTG AC | 700 | This study | |||
| A. braziliense | AbraProbe | 56FAM/TGA GCG CTA /ZEN/GGC TAA CGC CT/3IABkFQ/-3' | 119 | 200 | This study | |
| U. stenocephala | UncProbe | 5HEX/CAT TAG GCG /ZEN/GCA ACG TCT GGT G/3IABkFQ | 118 | 200 | This study | |
| Canine DNA | MAM F | CGACCTCGATGTTGGATCAG | 16S MtrRNA gene |
92 | 100 | (Hii et al. 2018) |
| MAM R | GAACTCAGATCACGTAGGACTTT | 100 | (Hii et al. 2018) | |||
| MAMPROBE | FAM/CCTAATGGT/ ZEN/ GCAGCAGCTATTAA/ LABKFQ | 200 | This study | |||
| Equine Herpes Virus | EHV FWD | GATGACACTAGCGACTTCGA | gB gene | 81 | 80 | (Bialasiewicz et al., 2009) |
| EHV-REV | TTTCGCGTGCCTCCTCCAG | 80 | (Bialasiewicz et al., 2009) | |||
| EHV PROBE | ROX/TTTCGCGTGCCTCCTCCAG/3IAbRQSp | 200 | (Bialasiewicz et al., 2009) |
+ Locked Nucleid Acid (LNA) basis