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Primers used for generating pGL4.23-ERRFI1 downstream enhancer reporter and pLKO.1-shERRFI1 construct
| Target | Forward | Reverse |
|---|---|---|
| EDE | 5’-CATGAGCTCTGGTATT GAGGTGACGCATTAG-3’ | 5’-GTCAAGCTTGGAACCG CAAGCAAGTAAAC-3’ |
| shERRFI1 | 5’-CCGGCTAGACCAGTAAA GCCAGATTCTCGAGAATCGGCTTTACTGGTCTAGTTTTTG-3’ | 5’- AATTCAAAAACTAGACC AGTAAAGCCAGATTCTCGAGAATCTGGCTTTACTGGTCTAG-3’ |
Abbreviation: EDE, ERRFI1 downstream enhancer.
