Figure 2:

Far-Western blotting studies using concentrated conditioned medium proteins to determine the presence of annexin A2 in these samples. (A) Conditions for preparing the control and WT PCSK9 (CircuLex) samples, electrophoresis using native conditions, and far-Western blotting analysis was described in Materials and Methods. A representative far-Western blot for annexin A2 (left blot) and a standard Western blot for follow-up probing of the same gel with a mouse anti-PCSK9 antibody (right blot) are shown. “Non-Cond” refers to samples prepared from an MITO+ medium that was not exposed to cells. (B) Standard Western blotting analysis for annexin A2 in medium and RIPA samples. Major protein bands have been indicated with arrows. The exposure time for each blot has been indicated.