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. Author manuscript; available in PMC: 2020 Jun 25.
Published in final edited form as: Int J Biomed Investig. 2020 Feb 27;3(1):123. doi: 10.31531/2581-4745.1000123

Figure 5:

Figure 5:

Affinity chromatography to isolate proteins secreted by C3A cells that interact with PCSK9. Twenty-five fractions were collected. (A) A typical Western blot for PCSK9 is shown. The primary antibody used in the Western blotting was mouse anti-PCSK9. “FT” stands for flow-through. “EB” stands for elution buffer. “MP” stands for medium protein. “WB” stands for washing buffer. (B) Total protein levels (ng/μL) determined using the BCA assay. (C) Relative PCSK9 levels (relative to the background signal) determined by quantitating the blot from (A).