Figure 3.

dPXXP cells exhibit increased AP-1 activity. EMSA for AP-1 (A) and NF -κB (B) binding. FL, dPXXP, dBAG, and control (Neo) cells were subjected to subcellular fraction. EMSA proceeded as described in the Materials and methods section with specific ³²P-labelled oligonucleotide probes. Luciferase activity from (C) p7×-AP-1-tk-LUC or (D) p4×-κB-tk-LUC was measured in the four cell lines. Results are presented as relative-fold induction over sham-treated control. Luciferase activity was normalized to β-galactosidase activity and results are presented as luciferase/β-galactosidase relative units. All results are the mean of at least three separate experiments done in duplicate (± SEM). ^∗pP < 0.05