Figure 5.

CCN1-sensitive invasion and adhesion of BAG3-expressing cells. (A) Expression of CCN1 with and without silencing in HeLa BAG3 cells subjected to invasion. (B) Bulk BAG3 (B3)-HeLa cells had a more robust invasion response to serum, and silencing of CCN1 reduced a greater proportion of B3 cell invasion. Results are mean ± SEM of four replicate experiments. (C) CCN1 matrix augments the adhesive ability of MDA435 dPXXP cells. MDA cells express less CCN1 in their basal or BAG3-transfected state than HeLa. Plates were coated with CCN1 ± collagen I. An additive effect of CCN1 and collagen was seen in dPXXP cells and increased adhesion observed with CCN1 or collagen I in the control and FL cells. (^∗p < 0.01; ^∗∗p < 0.004; ^∗∗∗p= 0.0006)