BAG3-dependent CCN1 signalling through integrin β1. (A) Total CCN1 and β1 integrin phosphorylation are reduced in FL MDA-435 cells. Neo, FL, and dPXXP MDA435 cells were grown for 72 h in the presence of either non-silencing siRNA (−) or CCN1 siRNA, and transferred to collagen IV-coated plates for adherence, lysis, and immunoblot. Incomplete silencing of CCN1 in dPXXP cells prevented loss of β1 phosphorylation and FAK phosphorylation. (B) Lysates used in A were immunoblotted for total integrin β1, total FAK, and β-tubulin. These results are representative of at least three replicates