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. Author manuscript; available in PMC: 2020 Jun 25.
Published in final edited form as: Eur J Cell Biol. 2010 Apr 1;89(7):564–573. doi: 10.1016/j.ejcb.2009.11.029

Fig. 3. GFP-VBP dimerization and DNA binding mutants by FRAP.

Fig. 3.

NIH3T3 cells were transiently transfected with plasmids containing mutations in the GFP-VBP B-ZIP domain and analyzed by FRAP as previously described. GFP-VBP (●) containing the basic region and leucine zipper region has the slowest recovery. Inhibition of VBP DNA binding by incubation with 100 μM NSC13746 (Δ) or co-transfection of GFP-VBP with mCherry-A-VBP (○) cause an increase in GFP-VBP recovery. Deletion of the basic region of VBP (GFP-VBP-LZ (x)) causes faster GFP-VBP recovery. The recovery of monomeric GFP-VBP-Proline (☐) is faster than all GFP-VBP dimers, but is slower than monomeric GFP.