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. Author manuscript; available in PMC: 2020 Jun 25.
Published in final edited form as: Eur J Cell Biol. 2010 Apr 1;89(7):564–573. doi: 10.1016/j.ejcb.2009.11.029

Fig. 7. Inhibition of B-ZIP DNA binding by NSC13746 or A-ZIP causes B-ZIP cytoplasmic localization.

Fig. 7.

Cellular transfections were performed as described previously. Images were taken using a Zeiss 510 confocal microscope and are representative of at least 3 independent experiments in which at least 10–15 different cells were imaged in each experimental condition. GFP-B-ZIP domains localize to the nucleus, with the exception of cFos which does not homodimerize. mCherry-A-ZIPs (in red) are localized throughout the cell. Incubating GFP-B-ZIPs with100 μM of NSC13746 caused a cytoplasmic localization for all B-ZIPs. A matrix of GFP-B-ZIP and mCherry-A-ZIP co-transfections show an A-ZIP-dependent cytoplasmic localization of the B-ZIP partner.