Skip to main content
. Author manuscript; available in PMC: 2020 Jun 25.
Published in final edited form as: Eur J Cell Biol. 2010 Apr 1;89(7):564–573. doi: 10.1016/j.ejcb.2009.11.029

Fig. 8. NSC13746 inhibits the growth of EWS-ATF1 containing clear cell sarcoma cells.

Fig. 8.

EWS-ATF1 and K562 leukemia cells were plated at 10,000 cells per well in 96 well plates. NSC13746 (active) or NSC13748 (inactive) compound was added to the wells in 1:2 serial dilutions starting at 100 μM. Cells incubated with the drugs in a 5% CO2 incubator at 37 °C for 96 h, after which CellTiter 96 Aqueous One Solution containing MTS was added and absorbance was recorded at 490 nm on a VersaMax microplate reader. NSC13746 caused ~25% growth inhibition in EWS-ATF1 cells while K562 leukemia cells (BCR-ABL) were unaffected. All reactions were performed in triplicates, and data is representative of at least two independent experiments.