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. Author manuscript; available in PMC: 2020 Jun 25.
Published in final edited form as: Eur J Cell Biol. 2010 Apr 1;89(7):564–573. doi: 10.1016/j.ejcb.2009.11.029

Fig. 9. NSC13746 inhibits colony formation of EWS-ATF1 cells.

Fig. 9.

Cells were plated at 20,000 cells/well (EWS-ATF1) and 5,000 cells/well (K562) in 24-well tissue culture plates on top of an agarose layer. After 24 h incubation in a 5% CO2 incubator at 37 °C, 25 μM antimony compound was added and plates were incubated for another 13 days. Colonies between 60 and 300 μm were counted using an Oxfor Optronix Gelcount colony counter. NSC13746 inhibited approximately 50% of colony formation in EWS-ATF1 cells while K562 (BCR-ABL) colony formation was unaffected. Plates were run in duplicate, and data is representative of at least two independent experiments.