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Yeast Hsp90 phosphorylation on serine (phos-Ser) and threonine (phos-Thr) residues. yHsp90-His6 was purified from yeast cells that were heat shocked at 39°C for 40 min or treated with 100 μM geldanamycin (GA) for 60 min. wild-type cells containing empty plasmid were used as negative control.
