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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2020 May 19;1862(9):183358. doi: 10.1016/j.bbamem.2020.183358

Table 1 – Identification of the modified SecA residues.

Forward primers used for recombinant PCR to introduce amino acid substitutions into E. coli SecA.

Structure Mutation(s) Primer
3JV2 G765C 5’-CTTCGAGAAATGCGTCATGCTGC-3’
2FSF A521C 5’-TGCTCGGTGGTAGCTGGCAGTGTGAAGTTGCCGCG-3’
1NL3 G605C 5’-GAGTATCCTGCATGATGCG-3’
G788C 5’-TCTGCGTCAGTGTATCCACCTG-3’