TABLE 2.

Phage adsorption assay

Strain	No. of spacers	Spacers acquired (5′–3′)	Targeted sequence on M102AD genome	% phage M102AD adsorptiona
WT	NAb	NA	N/A	84 ± 1
BIM4	7	sp3, AACGCTCTGATTTCGTGTTTGTGTTATCGCC	14311–14284	54 ± 4
		sp4, GTCAAGCATACGTATATATGCTTGTGCACT	25547–25518
		sp5, TGATGAAGTAAACCTCTTTTGTGAAAGGATT	24368–24338
		sp6, TTAGCGCGAGTGATGATGGGTTGGTAATTGCC	16891–16922
		sp7, CTCTAGCTTTATCTATTTTGATAAAGACAC	14862–14891
		sp8, GTACACTCTGCAACTAACCCATCGGCACCA	7587–7616
		sp9, CACGTCGAGTAAAATTGTACTAGCGCCTAA
		sp10, AACGCTCTGATTTCGTGTTTGTGTTATCGCC	14314–14284
		sp4, GTCAAGCATACGTATATATGCTTGTGCACT	25547–25518
BIM5	7	sp5, TGATGAAGTAAACCTCTTTTGTGAAAGGATT	24368–24338	35 ± 5
		sp6, TTAGCGCGAGTGATGATGGGTTGGTAATTGCC	16891–16922
		sp7, CTCTAGCTTTATCTATTTTGATAAAGACAC	14862–14891
		sp8, GTACACTCTGCAACTAACCCATCGGCACCA	7587–7616
		sp9, CACGTCGAGTAAAATTGTACTAGCGCCTAA
BIM6	2	sp11, AAATTTTATAGCATATGCGAATATTGTTGT	27729–27700	56 ± 5
		sp12, GTTAACCGCAAGCGTAAAGTTTGCATATGC	27896–27867
BIM7	4	sp13, AGAATTTTTCCATTCTTGCTCTTGGTTGGT	15737–15708	38 ± 3
		sp14, AGATGATAGTGACTTGTTTGCGGTAATTAA	3074–3103
		sp15, AACTCTAACACTGGCTATTACTGATAAGAC	15938–15967
		sp16, GAATTTTTCCATTCTTGCTCTTGGTTGGTT	15736–15707
BIM9	3	sp18, TGGTTTGCACATTTTTTTTCCTTCCTTTTT	26698–26669	20 ± 7
		sp19, TAAGATTACATTTTGCAAGTAATCTTTCTT	22856–22827
		sp21, GAATTGGGTTTTCCACAGTAGTAGCAAAGA	294–265
BIM24	5	sp38, GCTAGTGACGTTAAAGATTTTGATGATAAT	25233–25262	57 ± 12
		sp39, TAAACACAAAGAAGCTTTGCAAGCCGTCGG	10138–10167
		sp40, GCAGACAAAAGCTAAACAAGCTCTTGACTAT	8584–8614
		sp41, GCTACTCGTATGTTGGATGTTATCGACGCC	9878–9907
		sp1, CTCTTTTAGCAATTGTGAAAGGACGTAATT	24306–24335
BIM29	3	sp11, AAATTTTATAGCATATGCGAATATTGTTGT	27729–27700	5 ± 3
		sp51, ACGATGAAGTAAACCTCTTTTGTGAAAGGATT	24367–24338
		sp52, CTAAAACCGCAAGACACAGAGCCACAGGCT	4463–4492
BIM31	3	sp54, TTCTTTACTAGTAAAACTTCTGTACATTTA	196–167	17 ± 17
		sp51, ACGATGAAGTAAACCTCTTTTGTGAAAGGATT	24367–24338
		sp52, CTAAAACCGCAAGACACAGAGCCACAGGCT	4463–4492
BIM33	2	sp56, GCCTTTAGACGAATGTATCCAAAATGTATCC	22183–22213	9 ± 2
		sp55, CATTAAAAGCTATGCGCAATAAGGACTATGT	26770–26799
BIM34	6	sp57, GTTTTGGCTGTAACGTCTTTGACAACGCCG	5500–5471	9 ± 9
		sp58, TTTTGTAACTGCGTATCATCAGCGCTCGAG	16567–16538
		sp51, ACGATGAAGTAAACCTCTTTTGTGAAAGGATT	24367–24338
		sp59, CTTGCGATGTGGACAAATTGGGGCACGGTCA	6897–6927
		sp51, ACGATGAAGTAAACCTCTTTTGTGAAAGGATT	24367–24338
		sp52, CTAAAACCGCAAGACACAGAGCCACAGGCT	4463–4492
BIM35	4	sp60, TAAAGCGTTTTGGATTAACTGCGCTTTAGC	4783–4754	10 ± 10
		sp61, CGCATAGAGTTTTGAGAGGTGAAGAATGTTT	23905–23935
		sp51, ACGATGAAGTAAACCTCTTTTGTGAAAGGATT	24367–24338
		sp52, CTAAAACCGCAAGACACAGAGCCACAGGCT	4463–4492

^an = 2.

^bNA, not applicable.