TABLE 5.

Three viral genomes intermixed into the same reaction^a

Virus mix	Estimated viral genome copies/μl			Measured viral genome copies/μl
	HSV-1	HSV-2	VZV	HSV-1	HSV-2	VZV
1	1.1 × 106	5.6 × 103	2.8 × 102	1.2 × 106 (6)	1.0 × 104 (42)	1.9 × 102 (28)
2	1.3 × 104	5.9 × 104	3.4 × 101	1.3 × 104 (1)	6.3 × 104 (4)	1.4 × 101 (57)
3	1.2 × 103	6.2 × 103	4.2 × 104	1.1 × 103 (9)	5.1 × 103 (13)	2.7 × 104 (29)
4	1.2 × 102	4.5 × 101	4.2 × 103	1.1 × 102 (9)	7.2 × 101 (33)	3.3 × 103 (15)
5	1.1 × 105	4.6 × 101	3.6 × 104	1.1 × 105 (2)	8.9 × 101 (45)	3.0 × 104 (13)
6	1.2 × 102	5.9 × 104	4.2 × 104	1.6 × 102 (18)	4.9 × 104 (13)	4.2 × 104 (1)
	EBV	HCMV	KSHV	EBV	HCMV	KSHV
7	1.1 × 105	1.5 × 103	5.4 × 103	1.2 × 105 (5)	2.6 × 103 (37)	6.7 × 103 (15)
8	1.1 × 104	1.7 × 105	4.9 × 105	1.3 × 104 (12)	1.8 × 105 (4)	6.4 × 105 (20)
9	1.2 × 103	1.8 × 104	5.7 × 104	1.4 × 103 (10)	1.5 × 104 (11)	6.9 × 104 (14)
10	7.1 × 101	1.3 × 102	6.0 × 105	1.1 × 102 (31)	1.9 × 102 (26)	6.2 × 105 (2)
11	1.1 × 105	1.7 × 105	4.1 × 102	1.2 × 105 (6)	2.0 × 105 (12)	9.7 × 102 (57)
12	9.6 × 101	1.8 × 104	5.7 × 104	1.3 × 102 (22)	1.5 × 104 (14)	5.4 × 104 (3)
	HHV6-A	HHV6-B	HHV-7	HHV6-A	HHV6-B	HHV-7
13	1.9 × 103	6.2 × 103	1.0 × 102	1.7 × 103 (9)	7.1 × 103 (9)	1.3 × 102 (24)
14	1.9 × 103	6.1 × 102	1.0 × 102	1.7 × 103 (10)	4.6 × 102 (18)	1.1 × 102 (10)
15	9.3 × 101	6.1 × 103	5.2 × 101	1.2 × 102 (21)	5.6 × 103 (6)	3.6 × 101 (25)
16	1.3 × 103	6.1 × 101	5.2 × 101	1.4 × 103 (9)	1.2 × 102 (47)	3.6 × 101 (26)
17	4.5 × 100	7.9 × 101	1.0 × 102	1.1 × 101 (58)	5.2 × 101 (30)	1.1 × 102 (4)
18	1.3 × 103	6.1 × 103	5.2 × 101	1.9 × 103 (27)	1.1 × 104 (40)	4.9 × 101 (4)

^aOn the left are the estimated genome copies per dilution and on the right the copy numbers per microliter of DNA extract quantified by HERQ-9. In parentheses are the coefficient of variations (percent) between the estimated and measured copy numbers. Estimated and measured viral genome copies showed significant correlation (Pearson’s r = 0.996; P < 0.01).