FIG 3.
Excess folinic acid partially restores the growth of Candida albicans in the presence of methotrexate. A wild-type C. albicans strain (SC5314) was seeded at 1 × 104 cells/ml into YNB medium without folate or PABA with the indicated supplements and increasing concentrations of MTX. After 48 h of incubation at 30°C, growth was quantified as the OD600 value and expressed as a percentage of the value for the DMSO-treated control. The values presented here are the average of two replicates and are representative of two independent experiments.