FIG 8.
Efflux pump expression does not substantially alter Candida albicans susceptibility to methotrexate. C. albicans cdr1Δ/Δ (A), tac1Δ/Δ (B), or mdr1Δ/Δ (C) strains, or that overexpress CDR1 (A), TAC1 (B), or MDR1 (C) from the PTEF1 promoter, were subcultured to approximately 1 × 104 cells/ml into YNB medium at pH 5 or pH 7 supplemented with a range of MTX concentrations. After 48 h at 30°C, growth was quantified as the OD600 value and expressed as a percentage of the value for the untreated wells. The values presented here are the average of two replicates and are representative of two independent experiments. WT, wild type.