Figure 5. Proboscis motor neuron collection: arbors in brain (subesophageal zone).

(A) Motor neurons from the split GAL4 lines in Table 1, segmented to show arbors in isolation. Colors match muscles in previous figures. Most motor neurons are segmented from split GAL4 combinations in which their arbors are clearly distinguishable, with the exception that mn3M, 8, 11V, 12D and 13 are segmented from stochastic staining in order to separate them from nearby cells. Single neurons from stochastic staining are superimposed upon their mirror images to show bilateral arbors, for comparison with the neurons segmented bilaterally. mn10 is shown unilaterally, since it was never found bilaterally in any split GAL4 combination. Scale bar: 50 µm. (B) Motor neurons colored according to whether their dendrites are primarily dorsal (magenta) or ventral (green). Magenta: 10, 11D, 11V, 12D, 12V (13: not shown). Rest: ventral.

Figure 5—figure supplement 1. Location of motor neuron dendrites relative to other cell types in brain.

Motor neuron confocal images (green) from brains computationally aligned with brains stained for other cell types in magenta: (A–H) sweet taste inputs (Gr64f-GAL4), (J) eye mechanosensory inputs (VT017251-LexA), (K) bitter taste inputs (Gr66a-GAL4), and (L–N) Hugin neurons (HUGS3-GAL4). Frontal view (‘front’) or sagittal view (‘side’) of confocal maximum projections, except single sections where noted. Each cell type stained individually for GFP then aligned and overlaid. Gain and contrast adjusted. Scale bar: 50 µm.