Skip to main content
. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: FASEB J. 2020 Feb 26;34(4):4956–4969. doi: 10.1096/fj.201902649R

Figure 4. Contribution of the extracellular JMD1 of iR2 to the substrate selectivity of ADAM17.

Figure 4.

(A) Schematic of the domain organization of iR2 with a green arrow and box highlighting JMD1 (top panel). Amino acid sequence alignment of the JMD1 domain (shaded in green) of iR1 and iR2 with identical regions boxed (lower panel). (B) iR2 mutants (iR2-JMD1–1 to 1–3) were generated by replacing specific juxtamembrane amino acid residues with the corresponding iR1 residues. (C, D) Rescue experiments were conducted in iR1/2−/− mEFs to test the ability of iR2-JMD1–1 to 1–3 to restore stimulated ADAM17-dependent shedding of KL2 (iR2 selective) versus TGFα (iR1/2 selective). Results are shown as mean ± SEM (n=3). *P<0.05. (E) Western blot control for the expression levels of iR2-JMD1–1 to 1–3.