Figure 5.

Cell-based assays demonstrate that the MA^NOS mutant is defective for multi-round virus replication but exhibits WT levels of particle assembly and release. (a) CrFK cells were transfected with MA^WT, MA^NOS, and MA^G2A-containing clones. Virus replication was monitored by measuring RT activity in the supernatant. Two independent experiments are shown. (b) CrFK cells were transfected with MA^WT, MA^NOS, and MA^G2A-containing clones. Cell- and virion-associated p24(CA) levels were evaluated by western blotting. Similar results were obtained in multiple independent experiments. (c) Titration assays performed via co-transfection of CrFK cells with MA^WT and MA^G2A-containing clones at the indicated molar DNA ratios. Levels of virion-associated p24(CA) were evaluated by western blotting; blots were quantified in (d) from 4 independent experiments. Error bars indicate SDs.