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. Author manuscript; available in PMC: 2020 Nov 4.
Published in final edited form as: Nat Immunol. 2020 May 4;21(7):736–745. doi: 10.1038/s41590-020-0669-6

Extended Data Fig. 3. Effect of disulfiram on caspase-1 and caspase-11.

Extended Data Fig. 3

(a,b) Time course of caspase-1 (a) and caspase-11 (b) activity in the presence of indicated concentrations of disulfiram. Caspases (0.5 U) were incubated with disulfiram (at indicated concentrations for 1 hr before adding Ac-YVAD-AMC (40 μM)). (c,d) Dose response curve of disulfiram in the caspase-1 (a) and caspase-11 (b) activity assay. (e,f) Time course of caspase-1 (e) and caspase-11 (f) activity in the presence of indicated concentrations of disulfiram + Cu(II). Caspases (0.5 U) were incubated with disulfiram + Cu(II) (at indicated concentrations for 1 hr before adding Ac-YVAD-AMC (40 μM)). (g,h) Dose response curve of disulfiram + Cu(II) in the caspase-1 (e) and caspase-11 (f) activity assay. (a-h) n = 3 independent experiments. The mean ± s.e.m. is shown. Fluorescence intensity at 460 nm was measured after excitation at 350 nm.