Table 1. Selected primers for DMR validation using Zymo OneStep qMethyl Kit.

Probe ID	Gene Symbol	Gene Description	Forward Primer	Reverse Primer	Amplicon Size, bp
cg13078798	TGFBR3	Transforming growth factor, β receptor III	TGA GGA TGC ATT TGG ATG AG	AAG GCC TGT AGG CTG CTG TA	200
cg16055869	CXCL5	Chemokine (C-X-C motif) ligand 5	CTC TAC CGA GAG GGA CGT TG	AGG AGG AGC ATC TCC CAG AG	171
cg21951797	TNXB	Tenascin XB	GGA ACA CAA ATG ACC CGT AGA	AGC CCA CGT TTT TCT AGT GA	345
cg07139946			GGC TCA GTC AGA CCA GGA GA	AGG GCC AGT TTG ACT CCT TT	255
cg17982478	PLEKHA7	Pleckstrin homology domain contaning, family A member 7	CCC GAA CGC TCA GTA AAC C	CCA GCT GAA AAC TTG GGA AA	273
cg03133735	PITX2	Paired-like homeodomain 2	TAC TAT GCG TTG CCG ATT CC	AAA GAC CCC TGC TCC AAA AT	268
cg01733176			GTG TGG AAG GAG CTG GAC AT	ATT GCT TGC TTT GCT TGG AC	295
cg26500914 cg24498636	DAXX	Death-domain associated protein	GTA CCC CAT CCA CAC CTC AC	GGG CTG AGT GCT CTG ACT TT	263
cg05431670			GTC ACA GAG TTT CCG CCT TC	GTA GAA GCA CCG GGT GAA AA	268
cg16277169	TBX3	T-Box 3	GAA ACT GGA CGA AAG GTG GA	GGG CCA ACA GTT CTT CAA C	300
cg18161956			TCA GCA GCG AAA AGG TGA G	CAG AGA GGC TAA GGG GCT TT	300
cg09413529			TGC CCG TTG AAG AAC TGT TG	CAC CAT CTC GTC CAG CAC T	227
cg11246938			AGT GCT GGA CGA GAT GGT G	GAG AGC AAA GAG GAG CAT GG	201
cg09053536			GAA TTC AGT TTC GGG GAA CA	TTT GGC AAC TGA GGA GCA AT	322
cg13661397	NET1	Neuroepithelial cell transforming 1	GAT GCG CTC AGG AGT TAA GG	CAG CGA TCA GCC AAT CAG T	130