Fig. 3. FliD_cj amino-acid substitutions and their effect on motility.

a Location of the mutated residues on the FliD_cj structure, chosen due to their conserved nature and approximate location in the interacting interface. Adjacent subunits (Yellow, Orange) were chosen to represent the interaction of the N-terminus residues with that of the next subunit C-terminus and which might be important in the formation of the pentamer. The residues in the lower C-terminus were chosen to represent the interaction interface with flagellin upon flagellar elongation, as the conserved residues in that region which bind to the bottom subunit C-terminus might mimic the interactions with the flagellin monomer. b Motility assay results for point mutants represented as the mean percentage of the native complement strain, based on swarm diameter on soft agar. Controls (WT, deletion mutant and complement) are in orange. C-terminal mutants in light green and N-terminal mutants in purple. Error bars show standard deviation with N = 3. The corresponding data points are represented as dot plots. c Plot of the percentage flagella attachment to cells calculated from micrographs for each point mutant. Attached are colored green and unattached yellow. Source data are provided as a Source Data file.