Table 1.
Cryo-EM data collection, refinement and validation statistics.
| FliDcj (EMDB-10210) (PDB: 6SIH) | Filament (EMDB-10244) | |
|---|---|---|
| Data collection and processing | ||
| Microscope | Titan Krios | Tecnai Arctica |
| Magnification | 36232 | 53000 |
| Voltage (kV) | 300 | 200 |
| Camera | K2 Summit | Falcon III |
| Pixel size (Å) | 1.38 | 2.03 |
| Symmetry Imposed | D5 (full map), C5 (head map) | Helical (Rise: 7.25 Å, Twist: 65.4°) |
| Defocus range (µm) | −1.0 to −2.6 | −0.8 to −2.0 |
| Total dose (eÅ−2) | 41 | 45 |
| Number of micrographs | 1223 | 100 |
| Total particles used | 55967 | 71828 |
| Map Resolution (Å) | 4.7 (full map) 5.0 (head domain) | 8.6 (symmetrical) 27.2 (asymmetrical) |
| Refinement | ||
| Map sharpening B factor (Å2) | −285 | |
| Model composition | ||
| Non-hydrogen atoms | 37320 | |
| Protein Residues | 4880 | |
| B factors (Å−1) | ||
| Protein | 148 | |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.005 | |
| Bond angles (°) | 0.543 | |
| Validation | ||
| MolProbity score | 1.85 | |
| Clashscore | 8.36 | |
| Poor rotamers (%) | 0.00 | |
| Ramachandran plot | ||
| Favored (%) | 94.13 | |
| Allowed (%) | 5.87 | |
| Disallowed (%) | 0.00 | |