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. 2020 Jun 25;10:10332. doi: 10.1038/s41598-020-67117-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TEM and STXM analysis of an Aβ/ferritin aggregate formed following 48 hours of co-incubation. (a-c) TEM images of the Aβ/ferritin aggregate. Uniformly distributed electron dense fine particles can be seen in the highest magnification image in (c). STXM speciation dependent maps (d-f) and an iron L_2,3-edge X-ray absorption spectrum (g) from the aggregate. (d) Carbon K-edge protein map. (e) Iron L₃-edge map. (f) Composite image displaying protein (green) and iron (red) content of the aggregate. (g) Iron L_2,3-edge X-ray absorption spectrum from the iron content displayed in (e). The solid line for the spectrum corresponds to the best fit curve created by superposition of suitably scaled iron reference X-ray absorption spectra. (h) Reference iron L_2,3 edge X-ray absorption spectra for ferric (ferritin standard; blue), ferrous (FeCl₂; red), magnetite (Fe₃O₄; green) and elemental (Fe⁰; magenta) iron phases. Grey dashed and dotted lines indicate the energy positions of the principal Fe²⁺ and Fe³⁺ absorption peaks respectively. See also Supplementary Fig. S3 for additional ferritin iron L_2,3-edge X-ray absorption spectra.