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. 2020 Jun 25;10:10348. doi: 10.1038/s41598-020-67056-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alteration of Raw264.7 cells proliferation, cluster formation, and mobility by the 3D matrices. (A) The 3D matrices in different concentrations were measured their compressive modules by the unconfined compression test (n = 6, average ± standard deviation, (B). The scatter plot of cell proliferation in 3D matrices. The proliferation rates were calculated by counting the cell number from the Raw264.7 embedded in different 3D matrices, n = 3, 2^nd day: p = 0.1251, 6^th day: *p < 0.05, 8^th day: ***p < 0.001); (C) The cluster formation patterns. Optic images on the Raw264.7 cells embedded in different 3D matrices with MTT staining (blue arrows). (D) A diagram to illustrate the proposed experimental procedure for quantification of the migrated cells. (E) The absorbance measurement of the migrated Raw264.7 cells from the 3D matrices to culture medium, crystal violet staining at 3, 5, 9, and 12 days, n = 3, **p < 0.01.