Figure 4.

Genetic correction of RARG-S427L in iPSC-CMs decreases sensitivity to doxorubicin-induced cardiotoxicity. (a) Cell viability curve and (b) IC₅₀ of RARG-S427L/WT and its corrected isogenic control (WT/WT) assessed using Cell Titer-Glo after 24 h of doxorubicin incubation (n = 3). (c) Representative images showing staining for γH2A.X. Scale bar = 20 μm. (d) Relative to vehicle control fluorescent intensity of γH2A.X staining (n = 3). (e) Representative images showing reactive oxygen species (ROS) production, scale bar = 50 μm. (f) Relative to vehicle control fluorescent intensity of ROS production (n = 3). All experiments represent biological, independent replicates, each experiment was conducted in triplicates and are shown as mean ± s.e.m., *p < 0.05, ****p < 0.0001, t-test.