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. 2020 Jun 25;10:10374. doi: 10.1038/s41598-020-67319-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence localization of tricellulin in Ildr1 KO and WT mice large intestine and kidney. Representative immunofluorescence images showing angulin-2/ILDR1 (green) and tricellulin (red) antibody staining in each section of the colon for (A) WT mice and (B) Ildr1 KO mice. Scale bar 50 µm. Representative images showing tricellulin in the (C) thick ascending limb (using occludin and claudin 19 as markers) and (D) collecting duct (using occludin and AQP2 as markers) in the kidney of WT and Ildr1 KO mice.