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. 2020 Jun 25;11(6):487. doi: 10.1038/s41419-020-2688-5

Fig. 2. Analysis of fodrin, calpain, and calpastatin protein levels in cultured human SMA fibroblasts.

Fig. 2

Control (unaffected) and SMAII and SMAI patient fibroblast cell lines were plated in 35 mm culture dishes and maintained in the presence of supplemented MEME medium. Two days after plating, cell lysates were obtained and submitted to western blot analysis using anti-SMN antibody, anti-calpain 1 antibody, anti-fodrin antibody, or anti-calpastatin antibody. Membranes were reprobed with an anti-α-tubulin antibody. Graph values represent the expression of SMN, calpain, 150/145 fodrin product, or calpastatin vs. α-tubulin and correspond to the quantification of six independent experiments. Asterisks indicate differences using one-way ANOVA with Bonferroni multiple comparisons post-test (**p < 0.0003; ***p < 0.0001).