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. 2020 Jun 25;10:10300. doi: 10.1038/s41598-020-65648-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) The graphical representation of the changes observed in the fluctuation of the constituent residues between the RA bound and free MARK4 (B) 2-D eigenvector projection plot showing the differences between the flexibility of the two studied forms. (C) MMPBSA based generated curves highlighting the changes in the total, electrostatic and Van der Waals energies calculated between the MARK4 and RA.