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. 2020 Jun 25;10:10300. doi: 10.1038/s41598-020-65648-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell viability, protein expression and apoptosis studies of RA. (A) Cell viability studies of RA with MDA-MB-231, A549, and HEK-293 cells. Each data point shows the mean ± SD from n = 3. (B) Protein expression studies of MARK4 in MDA-MB-231 RA cells treated/vehicle-treated cells. Cells were treated with IC₅₀ concentration of RA for 48 h and total protein was isolated. The expression of the desired protein was accessed using immunoblotting. Raw images of immunoblotting are provided in the supplementary information. (C) Apoptosis induction studies of RA on MDA-MB-231 cells treated with IC₅₀ and 2 × IC₅₀ concentrations for 72 h using Annexin-V/PI staining. (C) Graphical representation of the percent apoptotic cells obtained from Annexin-V/PI staining for triplicate measurements ± SD.