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. 2020 Jun 25;11(6):488. doi: 10.1038/s41419-020-2693-8

Fig. 5. The effects of ATG5 siRNAs on growth, migration, and invasion of RBP1 overexpression OSCC cells in vitro.

Fig. 5

a Effective elimination of ATG5 mRNA in SCC15 cells were achieved, as determined by qRT-PCR analysis of total RNA preparations of these cells. b After transfection with control plasmid, RBP1 plasmid, or RBP1 plasmid with si-ATG5, the image of 1000 cells were severally plated in a six-well plate for 12 days; the average colony number of per well. c At 24, 48, and 72 h after transfection with control plasmid, RBP1 plasmid, or RBP1 plasmid with si-ATG5, cell growth was examined by the MTT assay. d The relative proportion of SCC15 cell cycle, after transfection with control plasmid, RBP1 plasmid, or RBP1 plasmid with si-ATG5. e The view of wound-healing migration assay; the average rate of SCC15 cells migration at 0, 12, and 24 h after treatment with control plasmid, RBP1 plasmid, or RBP1 plasmid with si-ATG5. Scale bar: 100 μm. f The results of the transwell assay of SCC15 cells at 24 h after treatment with control plasmid, RBP1 plasmid, or RBP1 plasmid with si-ATG5; the relative ratio of migrated and invasive cells per field was shown. Scale bar: 100 μm; *P < 0.05, **P < 0.01.