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. 2020 Jun 25;11(6):488. doi: 10.1038/s41419-020-2693-8

Fig. 7. CKAP4-induced autophagy in OSCC cells.

Fig. 7

a Effective overexpression and elimination of CKAP4 mRNA in SCC15 cells were achieved, as determined by qRT-PCR analysis of total RNA preparations of these cells. b Western blot analysis showing the expression levels of ATG5, BECN1, LC3-II, and β-actin in the cells transfected with control, CKAP4 plasmid, or si-CKAP4. Quantification of the amount of ATG5, BECN1, and LC3-II to the amount of β-actin for each of the indicated sample. c Electron microscopic examination showing autophagic vacuoles (arrows) in the cytoplasm in the cell transfected with control and CKAP4 plasmid. Scale bar: 2 μm. d Immunofluorescent staining showing the number of LC3 dot in the cell transfected with control, CKAP4 plasmid, or si-CKAP4. Cells were stained by indirect immunofluorescence using anti-LC3 antibody, and the number of LC3 dot per cell was quantified. Scale bar: 20 μm; *P < 0.05, **P < 0.01.