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. 2020 Jun 25;10:10351. doi: 10.1038/s41598-020-66977-9

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© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoblots of the optic nerve. (A) Analysis of p-Akt1 expression by using Western blotting. (B) Quantification of the protein bands of p-Akt1. Each value was normalized to GAPDH. Data are expressed as mean ± SD in each group (n = 6 in each group). The combination treatment induced higher p-Akt1 expression than treatment with meloxicam or low-dose G-CSF in the rAION model. *p < 0.05.