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. 2020 Jun 25;11(6):486. doi: 10.1038/s41419-020-2689-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Volcano plot of lncRNA expression in nonunion and bone normal fracture-healing tissue. b Heatmap of lncRNA expression in nonunion and bone normal fracture-healing tissue. Green, downregulated; red, upregulated. c qRT-PCR analysis of lncRNA expression in nonunion and bone normal fracture-healing tissue. BMSCs isolated from patient no. 1–24. d qRT-PCR analysis of lncRNA expression in BMSCs on the third day of osteogenesis. BMSCs isolated from patient no. 1, 2, 5, 12, 10, 14, 19, 20, 21, 22, 23, and 24. e qRT-PCR analysis of lncRNA ENST00000563942 expression in BMSCs during osteogenesis at 0, 1, 3, 7, and 14 days. BMSCs isolated from patient no. 1, 2, 5, 12, 10, 14, 19, 20, 21, 22, 23, and 24. f qRT-PCR analysis of lncRNA ENST00000563942 expression in the nucleus and cytoplasm of BMSCs. NC basal medium, OM osteogenic medium. BMSCs isolated from patient no. 1, 2, 5, 12, 10, 14, 19, 20, 21, 22, 23, and 24. g FISH analysis of lncRNA ENST00000563942 in BMSCs. All experiments were performed at least three times. Scale bar = 30 μm. The results are presented as mean ± SD. *p < 0.05, **p < 0.01.