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. 2020 Jun 25;11(6):486. doi: 10.1038/s41419-020-2689-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — qRT-PCR analysis of the CDH11 mRNA expression in BMSCs after transfection with Lenti-CDH11 (a) or pLKO.1-CDH11 (b). c Western blot analysis of CDH11 protein expression after transfection with Lenti-CDH11 or pLKO.1-CDH11. d ALP activity and Alizarin Red S staining after BMSCs transfected with Lenti-CDH11 or pLKO.1-CDH11 on the 7th and 14th day. Quantitative analysis of e ALP activity and f Alizarin Red S staining after BMSCs were transfected with Lenti-CDH11 and pLKO.1-CDH11 at the 7th and 14th day. qRT-PCR analysis of OCN (g), RUNX2 (h), and COL1A1 (i) mRNA expression in BMSCs after transfection with Lenti-CDH11 or pLKO.1-CDH11. j Western blot analysis of the beta-catenin protein expression after transfection with Lenti-CDH11 or pLKO.1-CDH11. k ALP activity and Alizarin Red S staining after BMSCs were transfected with Lenti-Vector and Lenti-ENST00000563492 along with pLKO.1-Vector or pLKO.1-CDH11 on the 7th and 14th day. Quantitative analysis of l ALP activity and m Alizarin Red S staining analysis after BMSCs. BMSCs were transfected with Lenti-Vector or Lenti-ENST00000563492 along with pLKO.1-Vector or pLKO.1-CDH11 on the 7th and 14th day. n Western blot analysis of the RUNX2 and beta-catenin protein expression after transfection with Lenti-Vector and Lenti-ENST00000563492. BMSCs isolated from patient no. 1, 2, 5, 12, 10, 14, 19, 20, 21, 22, 23, and 24. All experiments were performed at least three times. The results are presented as mean±SD. *p < 0.05, **p < 0.01.